Saturday, December 15, 2012

charles' Site - Fundraiser for Panthers Fencing | Media Sports ...

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Source: http://alvaortega.typepad.com/blog/2012/12/fundraiser-for-panthers-fencing-media-sports-recreation-food.html

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Source: http://charlesmurra8.multiply.com/journal/item/2726/Fundraiser-for-Panthers-Fencing-Media-Sports-Recreation-Food-...

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Video: Will Washington take on gun control? (cbsnews)

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Source: http://news.feedzilla.com/en_us/stories/politics/top-stories/270896928?client_source=feed&format=rss

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Team solves mystery associated with DNA repair

Friday, December 14, 2012

Every time a human or bacterial cell divides it first must copy its DNA. Specialized proteins unzip the intertwined DNA strands while others follow and build new strands, using the originals as templates. Whenever these proteins encounter a break ? and there are many ? they stop and retreat, allowing a new cast of molecular players to enter the scene.

Scientists have long sought to understand how one of these players, a repair protein known as RecA in bacterial cells, helps broken DNA find a way to bridge the gap. They knew that RecA guided a broken DNA strand to a matching sequence on an adjoining bit of double-stranded DNA, but they didn't know how. In a new study, researchers report they have identified how the RecA protein does its job.

"The puzzle for scientists has been: How does the damaged DNA look for and find its partner, the matching DNA, so that it can repair itself?" said University of Illinois physics professor Taekjip Ha, who led the study. "Because the genomic DNA is millions of bases long, this task is much like finding a needle in a haystack. We found the answer to how the cell does this so quickly."

The research is described in a paper in eLife, a new open-access journal supported by the Howard Hughes Medical Institute (HHMI), the Max Planck Society and the Wellcome Trust. Ha is an HHMI investigator. The National Science Foundation provided primary funding for this work.

DNA repair is vital to health, vitality and longevity. Disruptions of the process can lead to the early onset of diseases associated with aging or cancer in animals. The breast cancer mutation known as BRCA2, for example, disrupts a gene involved in loading Rad51 (the human equivalent of RecA) onto a broken DNA strand to begin the process of repair.

Previous studies have shown that in bacteria, RecA forms a filament that winds itself around a broken, single strand of DNA. Like a matchmaker trying to find a partner for an unpaired dancer, it scours the corresponding DNA strands for a sequence that will pair up perfectly with the broken strand. Once it finds the sequence, the broken strand steps in and chemically bonds to its new partner, displacing one of the unbroken strands (which eventually pairs with the other broken strand). This elaborate molecular square dance allows the cell to go back to the work of duplicating its genome. Each broken strand now is paired with an unbroken one, and uses the intact strand as a template for replication. (Watch an animation about this process.)

"If a break in DNA occurs, you have to repair it," Ha said. "We wanted to know how RecA helps the DNA find a sequence complementary to it in the sea of genomic DNA, and how it does it so quickly."

To answer this question, the researchers made use of fluorescence resonance energy transfer (FRET) to observe in real time the interaction of the RecA protein and the DNA. FRET uses fluorescent molecules whose signals vary in intensity depending on their proximity to one another. By labeling a single DNA strand bound by RecA and putting a different fluorescent label on a stretch of double-stranded DNA, the researchers could see how the molecules interacted with one another.

The team determined that RecA that is bound to a broken, single-stranded DNA molecule actually slides back and forth along the double-stranded DNA molecule searching for a match.

"We discovered that this RecA filament can slide on double-stranded DNA for a span of sequences covering about 200 base pairs of DNA," Ha said. "This is how one strand of DNA can be exchanged with another from a different DNA duplex. That's the process called 'recombination.' "

The discovery explains how DNA repair can occur so quickly, Ha said.

"We did a calculation that found that without this kind of process that we discovered, then DNA repair would be 200 times slower," he said. "So your DNA would not be repaired quickly and damage would accumulate, possibly leading to serious diseases."

The research team included graduate students Kaushik Ragunathan and Cheng Liu. Ha is an affiliate of the Institute for Genomic Biology and a co-director of the NSF Center for the Physics of Living Cells at Illinois.

###

University of Illinois at Urbana-Champaign: http://www.uiuc.edu

Thanks to University of Illinois at Urbana-Champaign for this article.

This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.

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Source: http://www.labspaces.net/125940/Team_solves_mystery_associated_with_DNA_repair

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Friday, December 14, 2012

Why 12/12/12 Is a Lucky Day for Love | YourTango

Love on 12/12/12
Could this be your lucky day?

Is today the best day for love?

Today's date holds a lot of significance: Another triple day, a huge benefit concert for Hurricane Sandy, oh, and thousands of weddings! Remember?10/10/10 and 11/11/11? Just like in recent years with repeating dates, many couples are tying the knot on this once-in-a-lifetime day in hopes of gaining good karma.

More from YourTango: 35 Gifts For The Men Who Have Everything

According to a David's Bridal survey, 7,500 American couples will wed today. In comparison, only 485 got married on 12/12/11. That means tons of trips to Vegas, countless discounted wedding packages, and an overflow of Elvis impersonators.

But why so lucky? For starters, this iconic day is considered to be the last major numerical event (January 1, 2101 is the next) of our lifetimes, so many people want to be apart of a historical moment on their big day.?

Based off Western numerology, 1 and 2 make a powerful pair that balances each other out ? 1 is masculine, while 2 is feminine. One also represents new beginnings and 2 symbolizes union, according to MSN Glo. Sounds like a good relationship to us!

According to Chinese numerology, the number 12, which brings together the ying (2) and the yang (1), brings harmony.

Some also say 12 symbolizes completeness, from the months in a year to the number of hours from day to night. A dozen roses sounds pretty fulfilling to us too!

Couldn't make it to the altar today? There's still time to plan for another wedding anniversary that no one will forget: 11/12/13.

More from YourTango: Boudoir Holiday Photos: The Perfect Holiday Gift?

Did you attend a 12/12/12 wedding or know someone who did??What do you think about iconic wedding dates?

More juicy stories on YourTango:

Source: http://www.yourtango.com/2012167590/121212-lucky-day-love

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Merkel coalition stymied by opposition veto on tax cuts

BERLIN (Reuters) - Chancellor Angela Merkel's center-right coalition has suffered a humiliating defeat by the center-left opposition when lawmakers threw out a plan to cut income taxes by 6 billion euros.

Rejection of the plan, which had been seen as an election-year gift to voters, showed the waning strength of Merkel's coalition nine months before the poll, analysts said.

Despite promises of cutting taxes when it took power in 2009, internal dissent within the center-right government at first blocked the initiative and later it was stymied by the opposition.

"This government hasn't accomplished a thing when it comes to tax reform in the last three years and it doesn't look like they'll get anything done in the next year either," said Gero Neugebauer, political scientist at Berlin's Free University.

"They got off on the wrong foot and never got it right. It shows how weak they really are," he added.

The setback came just before midnight on Wednesday when the center-left opposition Social Democrats (SPD) and Greens used their veto powers in the Bundesrat, the upper house of parliament representing the federal states, to block the plan.

The SPD and Greens argued there was no scope for tax cuts, especially because they would reduce revenues to states and towns. They said they would have backed tax cuts for ordinary earners if the coalition had raised taxes on higher incomes.

A parliamentary mediation committee, made up of members of both the upper house and the lower house Bundestag spent five hours on Wednesday evening exploring scope for an agreement. But it failed because the SPD and Greens stuck to their guns.

In a further sign of its weakness, the coalition saw a tax deal with Switzerland blocked by the mediation committee. It would have required Swiss banks to levy a punitive charge on an estimated 150 billion Swiss francs ($160 billion) in undeclared funds stashed away by Germans in Swiss accounts [ID:nL5E8NCH6H]

WASTED CHANCES

"The coalition didn't use the chance when it had a majority in 2009 and 2010," said Gerd Langguth, a political scientist at Bonn University. "They wasted time."

Merkel's center-right coalition had a majority in the upper house when it took office in 2009 but Merkel hesitated when the idea of tax cuts was first aired and the coalition lost its upper house majority in 2010.

The coalition was widely criticized at the start in 2009 for cutting taxes for hotel operators, with the Free Democrat (FDP) junior coalition partners plunging in opinion polls after that to around 3 percent from 14.6 percent in 2009.

The FDP had hoped this 6-billion euro tax cut would help to restore some of its lost credibility with voters but the coalition itself was long divided about the tax cut plans.

The Christian Social Union (CSU), the Bavarian sister party to Merkel's Christian Democrats (CDU), had long resisted tax cuts. The public wrangling lasted months and earned Merkel's government the nickname "chaos coalition" in the media.

The coalition agreed to cut income taxes by 2 billion euros in 2013 and another 4 billion euros in 2014.

Finance Minister Wolfgang Schaeuble had hoped the tax cuts would have leveled out the effects of "cold progression", also known as "bracket creep".

These terms describe what happens when tax thresholds are not adjusted for inflation. Workers getting pay rises sometimes see net pay actually fall because they have entered a higher tax bracket.

Thanks to the "bracket creep" in the tax code, the treasury takes in three billion euros in extra revenues each year.

Merkel will seek a third term next September. Her conservatives are well ahead of opposition parties in opinion polls but her allies the FDP may fail to get back into parliament, making a 'grand coalition' with the SPD possible.

(Editing by Gareth Jones and Stephen Nisbet)

Source: http://news.yahoo.com/merkel-coalition-stymied-opposition-veto-tax-cuts-183236625.html

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Wednesday, December 12, 2012

Facebook says some users briefly kept from site

SAN FRANCISCO (AP) ? Facebook says some users were briefly unable to access the site because of a technical glitch.

The social networking site with over 1 billion users worldwide says on Monday it made a change to its domain name system setup. The change resulted in some users temporarily not being able to reach the site.

The problem was detected and the site is now fully back.

A DNS acts like a phone book for the Internet, translating a domain name like facebook.com into a number that points to a specific host computer.

Facebook did not say how many users were affected or for how long. Some technology blogs say the problems lasted about 20 minutes.

Source: http://news.yahoo.com/facebook-says-users-briefly-kept-021815799.html

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Tuesday, December 11, 2012

Real Politix ? Navigating The World Of Real Estate Buying

TIP! If you are considering the purchase of a house, check the neighborhood out through the online sex offender registry to ensure there are none located nearby. A real estate agent is not obligated to let you know if there is a sex offender living next door, or anywhere in the neighborhood.

Purchasing a piece of real estate can change your life. However, this stress can be relieved by having the best knowledge. Reading these tips, you can become well-informed and have a positive buying experience.

Former Clients

TIP! It is important to clarify with the landlord before signing the lease, who is responsible for the upkeep of any garden on the property. Sometimes rentals require that you handle it yourself, or for you to hire a gardener or yard person.

Each agent should connect with former clients on special occasions to maintain the relationship. When your former clients receive a holiday card from you, they will remember how helpful your were when they purchases their home. When you are done with your greeting, make sure they know that you are working as a referral and would love for them to recommend you to anyone they know.

TIP! If you are planning on having a family, look for a house with enough space. Don?t neglect safety issues as well, especially if there is a swimming pool or stairs in the home you?re considering buying.

If you want to have a good return on your property, try to do some remodeling. You?ll see an immediate increase in the value of your home. In fact, the value of the home may even go up more then you actually invested in it.

TIP! Get a professional inspector to look over any home you are thinking about making an offer on. If you don?t you could wind up with a home that will need a lot of repairs.

When you find a house that you want to purchase, hire an inspector to check out the house. Hiring a professional is important, regardless of cost. If you have a friend or family member perform an inspection, they may miss something, requiring you to take an expensive fall.

TIP! You need to make sure that you fully understand all of the terms that will be on the mortgage loan if you are thinking about buying a new house. Understanding how monthly payments are calculated and how interest is accrued is essential to knowing whether your budget can withstand the additional expenses.

Houses that need some ?tender loving care? are priced less. This permits you to save your money on the purchase price, and you have time to work on your home at your own pace. If you happen to get the property for a great price, you can also add a significant amount of equity by making the right improvements. Concentrate on placing emphasis on the home?s possibilities instead of its flaws. A little fix up work can transform an ugly facade into your dream home!

TIP! When looking to purchase a home, if you currently own a car or plan to buy one, confirm that there is ample parking available in the location you are considering. If the home has no driveway, good street parking is essential.

Before you run out and purchase a property simply because you like the way it looks, you should first stand back and examine the home as a whole. You should only consider buying a home based on it?s construction and shape. If you focus too much on appearances, you will end up overlooking the important features and perhaps flaws of the home.

TIP! You should put off a home purchase if you have any uncertainty about your current job or employment outlook. Once your signature is on mortgage documents, you will be responsible for your payments, regardless of changes in your personal life.

When considering purchasing a house that comes with a great view, it is important to refrain from paying an increased cost simply for the view. Even though you value the view highly, when it comes time for you to resell the house, your buyer might not think so. Pay for the home and view you love, but don?t pay too much.

Term Goals

TIP! You most likely will want to work with an attorney that is familiar with foreclosures if that is the type of property you are looking to purchase. You need good legal representation because complications during foreclosure deals often arise.

Clearly delineate your goals when you are considering whether or not to invest in the real estate market. Do you want to work on achieving your short-term goals or your long-term goals? If that investment doesn?t match your goals, don?t consider it. People make the mistake of not ensuring their goals are met and wind up being unhappy with their investment.

TIP! Even if your first offer is not accepted, keep hope that there may be methods to make the home more affordable. Sellers will often cover repairs or closing costs, in order to help a buyer afford the property.

Maintain contact with real estate investors. There are always properties available, and not every property is a good match for every investor. This can help you find that hidden gem that hasn?t been purchased yet.

TIP! Don?t just research the property you are interested in; research the surrounding neighborhood, as well. If the neighborhood is not to your liking, you could be miserable very quickly with your purchase.

When deciding to purchase a large and commercial piece of real estate, make sure you get a trustworthy partner. When you have a partner who has a good financial standing and reputation, it is much easier to quality for the loan needed to purchase the property. Having a good partner will help you with a down payment, while also making the commercial loan qualification process much easier.

TIP! When you choose a real estate agent, ask how many years they have been involved in the community you are interested in. If the agent is fairly new to the area, they will not know very much about community issues, streets and neighborhoods.

The tips that were laid out in this article will help you to navigate the home buying process in the easiest way possible. Preparing yourself is the best way to make a successful investment.

Source: http://www.realpolitix.com/navigating-the-world-of-real-estate-buying/

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